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한국식품과학회지
→ 한국식품과학회지2014 ; 46(3): 328-333
Screening of Lactic Acid Bacteria for Strong Folate Synthesis and Optimization of Fermentation
고엽산 생산능의 유산균 탐색 및 발효 조건 최적화
Kyung Min Du1, Se Jin Park1, Myung Soo Park2, and Geun Eog Ji1,3*
1Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University 2Department of Hotel Culinary Arts and Tourism, Yeonsung University 3Research Institute, Bifido Inc.
두경민1·박세진1·박명수2·지근억1,3*
1서울대학교 식품영양학과 식품미생물 연구실, 2연성대학교 식품과학부 호텔조리과, 3(주)비피도
ABSTRACT
Folate is a water-soluble vitamin B that is required for the synthesis of amino acids and nucleic acids. It plays an important role in cell division and cell growth in several living organisms. The purpose of this study was to screen strong folate-synthesizing bacteria and to optimize their culture conditions for folate production. Folate production was quantified by microbiological assays by using folate-dependent strain Lactobacillus rhamnosus KCTC 3237. Folate derivatives were identified by LC-MS/MS. Of the 65 strains of bifidobacteria and lactobacilli tested, L. plantarum Fol 708 demonstrated the greatest ability to produce folate. Its optimal pH for folate production was 5.5 in a pH-controlled, labscale fermenter. Coculturing L. plantarum Fol 708 with L. brevis GABA 100 in a milk medium enhanced the level of folate produced in comparison to culturing L. plantarum Fol 708 alone.
KEYWORD
folate, microbiological assay, fermented-food, lactobacilli, co-culture
한국식품과학회지 2014 Jun; 46(3): 328 - 333
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