→ 한국식품과학회지2021 ; 53(3): 304-312
Immuno-stimulating and anti-metastatic activities of the polysaccharides isolated from Angelica gigas
참당귀로부터 분리한 다당의 면역증진 활성과 항전이 활성
Seung-U Son1 and Kwang-Soon Shin1,*
1Department of Food Science and Biotechnology, Kyonggi University
손승우1 · 신광순1,*
1경기대학교 식품생물공학과
The present study aimed to develop new physiologically active ingredients from Angelica gigas. The polysaccharides purified from A. gigas, AGE-2c-I, showed potent anti-complementary activity in a dose-dependent manner. C3 activation products were identified through crossed immuno-electrophoresis using anti-human C3 antibodies and the anticomplementary activity of AGE-2c-I under Ca++-free conditions suggests that AGE-2c-I may induce complementary activation via both alternative and classical pathways. In addition, AGE-2c-I augmented the production of various cytokines, such as interleukin (IL)-6, IL-10, IL-12, and tumor necrosis factor-α, by peritoneal macrophages. Furthermore, intravenous (i.v.) administration of AGE-2c-I dose-dependently enhanced natural killer cell cytotoxicity against YAC-1 lymphoma. In experimental lung metastasis, prophylactic i.v. administration of AGE-2c-I inhibited lung metastasis by 58% at 100 μg/mouse. From the above results, we suggest that AGE-2c-I purified from A. gigas has potent immune systemstimulating activities, and is a potentially promising food ingredient beneficial to human health.
1Department of Food Science and Biotechnology, Kyonggi University
Angelica gigas, complement system, macrophage, NK cell, metastasis
한국식품과학회지 2021 Jun; 53(3): 304 - 312