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Food Science and Biotechnology
→ Food Science and Biotechnology 2021 ; 30(5): 735-744
Isolation, characterization, and genomic analysis of the novel T4-like bacteriophage ΦCJ20
Jaegon Kim1 • Jong Pyo Chae2 • Gyeong-Hwuii Kim1 • Jae-Won Kim2 • Na-Gyeong Lee1 • Jun-Ok Moon2 • Sung-Sik Yoon1
1 Department of Biological Science and Technology, Yonsei University, 1 Yeonsedae-gil, Heungeop-myeon, Wonju-si, Gangwon-do 26493, Republic of Korea 2 Institute of Biotechnology, CJ CheilJedang, Suwon, Gyeonggi-do 16495, Republic of Korea
ABSTRACT
Pathogenic Escherichia coli infections have been consistently reported annually. The basic characteristics and genome of the newly isolated ΦCJ20 from swine feces was analyzed. To determine basic characteristics, dotting assays and double-layer agar assays were conducted. Bacteriophage particles were analyzed via transmission electron microscopy. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis was performed to determine the sizes of major structural proteins. The complete genome of the phage was analyzed. Bacterio-phage particles were identified as Myoviridae, with a head measuring 110.57 ± 1.89 nm and a contractile tail measuring 107.97 ± 3.20 nm and were found to infect E. coli. Major structural proteins of ΦCJ20 showed two well-pronounced bands of approximately 53.6 and 70.9 kDa. The genome size of ΦCJ20 was 169,884 bp, and 118 of 307 open reading frames were annotated. This study provides a baseline for the development of E. coli infection treatment strategies.
KEYWORD
Escherichia coli · Bacteriophage · Genome annotation · Antibiotics substitution · Food safety
Food Science and Biotechnology 2021 ; 30(5): 735-744
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