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Food Science and Biotechnology
→ Food Science and Biotechnology 2018 ; 27(1): 251-259
Individual and combined cytotoxicity assessment of zearalenone with ochratoxin A or α-zearalenol by full factorial design
N. Zheng1,2, Y. N. Gao1,2, J. Liu4, H. W. Wang1,3, J. Q. Wang1,2
1Ministry of Agriculture Laboratory of Quality and Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, People’s Republic of China, 2State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, People’s Republic of China, 3College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, People’s Republic of China, 4China National Research Institute of Food and Fermentation Industries, Beijing 100027, People’s Republic of China
ABSTRACT
The combined mycotoxins zearalenone (ZEA) with ochratoxin A (OTA) or α-zearalenol (α-ZOL) are frequently found together in milk. Toxicological data concerning the combined effects of these mycotoxins are sparse. In present study, individual and combined ZEA, OTA and α-ZOL caused cytotoxicity and oxidative damage, including reductions in intracellular superoxide dismutase and glutathione peroxidase activities and glutathione content, along with increases in malonaldehyde content on human Hep G2 cells after 48 h of exposure. Among individual mycotoxins, OTA had the greatest cytotoxic effect followed by α-ZOL. Compared with individual mycotoxins, combinations produced more serious negative effects, more importantly, ZEA + OTA was antagonistic for these effects, whereas ZEA + α-ZOL was antagonistic at low concentrations, but synergistic at high concentrations of ZEA, which were evaluated by 3 × 3 full factorial analysis and estimated marginal means plots. Our results also demonstrated a significant correlation between cytotoxicity and oxidative damage in response to these combinations.
KEYWORD
Zearalenone, Ochratoxin A, α-Zearalenol, Combined effect, Full factorial design
Food Science and Biotechnology 2018 ; 27(1): 251-259
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