HOME | LOGIN | MEMBER JOIN | CONTACT US | ENGLISH

Food Science and Biotechnology
→ Food Science and Biotechnology 2018 ; 27(1): 9-17
Fabrication of an electrochemical biosensor with ZnO nanoflakes interface for methylglyoxal quantification in food samples
Amudha Jayaprakasan1,2, Amarnath Thangavel1,2, Lakshmishri Ramachandra Bhat1,3, Manju Bhargavi Gumpu1,3, Noel Nesakumar1,3, K. Jayanth Babu1,2, Srinivasan Vedantham1,2, John Bosco Balaguru Rayappan1,3
1Centre for Nanotechnology & Advanced Biomaterials (CeNTAB), SASTRA University, Thanjavur, Tamil Nadu 613 401, India, 2School of Chemical & Biotechnology, SASTRA University, Thanjavur, Tamil Nadu 613 401, India, 3School of Electrical & Electronics Engineering, SASTRA University, Thanjavur, Tamil Nadu 613 401, India
ABSTRACT
Increased consumption of fried foods such as grilled chicken contains elevated levels of methylglyoxal (MG), which is associated with diabetes mellitus. Hence, in this work, glyoxalase 1(GLO 1) based, zinc oxide (ZnO) flakes interfaced mediator free electrochemical biosensor was developed to detect MG in grilled chicken. ZnO flakes were synthesized by direct precipitation method. X-ray diffractometer and field emission scanning electron microscope were used to study the structural and morphological characteristics of ZnO flakes. The immobilization of GLO 1 on Pt/ZnO flakes modified electrode was confirmed by Fourier transform infrared spectroscopy. Cyclic voltammetric and amperometric studies were carried out using Pt/ZnO flakes/GLO 1 working electrode. The developed biosensor exhibited linear range of 0.6–2.0 µM, sensitivity of 0.281 µA µM-1, LOD of 9 nM with a response time of <4 s and shelf life of 18 days (89%).
KEYWORD
Biosensor, ZnO flakes, Human glyoxalase 1, Methylglyoxal, Cyclic voltammetry, Amperometry
Food Science and Biotechnology 2018 ; 27(1): 9-17
List