→ 한국식품과학회지2017 ; 49(06): 668-675
Protective effects of Aruncus dioicus var. kamtschaticus extract against hyperglycemic-induced neurotoxicity
포도당 처리로 유도된 뇌신경세포 독성에 대한 눈개승마 추출물의 보호효과
Su Bin Park#, Uk Lee1,#, Jin Yong Kang, Jong Min Kim, Seon Kyeong Park, Sang Hyun Park, Sung-Gil Choi, and Ho Jin Heo*
Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University 1Division of Special Purpose Tree, National Institute of Forest Science
박수빈·이 욱1,†·강진용·김종민·박선경·박상현·최성길·허호진*
경상대학교 응용생명과학부(BK21 plus)·농업생명과학연구원, 1국립산림과학원 특용자원연구과
To assess the physiological effects of Aruncus dioicus var. kamtschaticus extract on cytoxicity of a neuronal cell line, antioxidant activity, and neuroprotection against intensive glucose-induced oxidative stress were quantitated. Compared to the other fractions, the ethyl acetate fraction of Aruncus dioicus var. kamtschaticus (EFAD) showed the highest total phenolics and flavonoids. The 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assay and malondialdehyde inhibitory effect test confirmed the superior antioxidant activity of EFAD. Moreover, EFAD also decreased the intracellular ROS level and suppressed neuronal cell death against intensive glucose- or H2O2-induced oxidative stress. Additionally, assessment of α-glucosidase and acetylcholinesterase inhibitory activities revealed that EFAD was an effective inhibitor of α-glucosidase and acetylcholinesterase. Finally, high-performance liquid chromatography analysis identified caffeic acid as the main ingredient of EFAD. Overall, these results suggest that the EFAD is a good natural source of biological compounds that counteract hyperglycemic neuronal defects.
antioxidant activity, Aruncus dioicus var. kamtschaticus, caffeic acid, hyperglycemic neurotoxicity
한국식품과학회지 2017 Dec; 49(06): 668 - 675