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한국식품과학회지
→ 한국식품과학회지2017 ; 49(06): 610-616
Overproduction of a γ-glutamyltranspeptidase from Bacillus amyloliquefaciens in Bacillus subtilis through medium optimization
배지최적화를 통한 재조합 바실러스 서브틸리스에서 바실러스 아밀로리퀴파시엔스 유래 γ-글루타밀펩타이드전달효소의 대량생산
Hye-Bin Cho, Jetendra Kumar Roy, Wu-Jin Park1, Byoung-Oon Jeon1, and Young-Wan Kim*
Department of Food and Biotechnology, Korea University 1R&D Center, Nongshim Co.
조혜빈·제텐드라 쿠마르 로이·박우진1·전병운1·김영완*
고려대학교 과학기술대학 식품생명공학과, 1(주)농심 중앙연구소
ABSTRACT
γ-Glutamyltranspeptidase (GGT, EC 2.3.2.2) transfers γ-glutamyl moiety from glutamine to amino acids or peptides and hydrolyzes glutamine to glutamate and ammonia. In order to overproduce γ-glutamyltranspeptidase from Bacillus amyloliquefaciens (BAGGT), the encoding gene was cloned and expressed in Bacillus subtilis. The productivity of BAGGT in Bacillus subtilis was improved by 42-fold by using a dual-promoter system that was generated by combining promoters from B. subtilis α-amylase and BAGGT genes. Through optimization of medium composition by Plackett-Burman design and central composition design, BAGGT was produced at 18.3×107 U/L of culture in the optimized medium. Compared to previously used Luria-Bertani medium, the optimized culture medium (15 g/L molasses, 60 g/L corn steep liquor, 6 g/L yeast extract, 4 g/L NaCl, 6 g/L K2HPO4, and 2 g/L KH2PO4), resulted in a 4.3-fold increase in production of BAGGT.
KEYWORD
Bacillus subtilis, dual-promoter system, γ-glutamyltranspeptidase, medium optimization
한국식품과학회지 2017 Dec; 49(06): 610 - 616
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