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Food Science and Biotechnology
→ Food Science and Biotechnology 2017 ; 26(5): 1281-1290
Extraction of caffeine and catechins using microwave-assisted and ultrasonic extraction from green tea leaves: an optimization study by the IV-optimal design
Vahid Ghasemzadeh-mohammadi1, Bahman Zamani1, Maryam Afsharpour2, Abdorreza Mohammadi1
1Department of Food Science and Technology, Faculty of Nutrition Science, Food Science and Technology, National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran, 2Department of Inorganic Chemistry, Chemistry and Chemical Engineering Research Center of Iran, Tehran, Iran
ABSTRACT
In this research, optimal conditions for extraction of caffeine and polyphenols were established from Iranian green tea leaves. In the first step, caffeine was extracted with efficacy about 86% versed to 4.5% of EGC + EGCG. The EGCG + EGC was extracted from partially decaffeinated green tea leaves through microwave-assisted extraction (MAE) and ultrasound-assisted extraction (USE) with efficiency levels of 95 and 85%, respectively. The best results for the MAE process were obtained with 7.8 min and three number of extraction cycles and for the USE process were as followed: time 57 min, temperature 65 ℃, and the number of extraction cycles 3. The total phenol content values at the best conditions of MAE and the USE processes were 125 ± 5 and 96 ± 6 mg gallic acid/g DW. The 50% inhibition (IC50) on 1,1-diphenyl-2-picrylhydrazyl (DPPH) were 56 and 66 mg/g of phenol for the MAE and USE processes.
KEYWORD
Catechins extraction, Tea, Microwave, Ultrasound, RSM
Food Science and Biotechnology 2017 ; 26(5): 1281-1290
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