→ 한국식품과학회지2017 ; 49(03): 258-264
Comparison and validation of rutin and quercetin contents according to the extraction method of tartary Buckwheat (Fagopyrum tataricum Gaertn.)
쓴메밀 종자의 추출방법에 따른 루틴 및 퀘세틴 함량 비교
Su Jeong Kim, Hwang Bae Sohn, Geum Hee Kim, Yu Young Lee1, Su Young Hong, Ki Deog Kim, Jeong Hwan Nam, Dong Chil Chang, Jong Taek Suh, Bon Cheol Koo, and Yul Ho Kim*
Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration 1Department of Central Area, National Institute of Crop Science, Rural Development Administration
국립식량과학원 고령지농업연구소, 1국립식량과학원 중부작물부
The stability and accuracy of ultra-performance liquid chromatography (UPLC) used for evaluating the contents of rutin and quercetin in tartary buckwheat (Fagopyrum tataricum Gaertn.) seeds extracted by seven different extraction methods were determined. The seven extraction methods were reflux extraction (RE), ultra-sonification extraction (UE), stirrer extraction (SE), RE after UE (UE+RE), RE after SE (SE+RE), UE after SE (SE+UE), and RE with UE after SE (SE+UE+RE). Among the seven extraction methods used, RE yielded comparatively higher contents of rutin (2,277 mg/100 g) and quercetin (158 mg/100 g) than those yielded by other six extraction methods. The intra-day repeatability and inter-day precision of RE was 0.4-3.2% considering relative standard deviation (RSD), while accuracy was 88.8-102.4%. Therefore, RE with UPLC would be a rapid, accurate, and stable method for analyzing rutin and quercetin contents in tartary buckwheat.
reflux extraction; rutin; quercetin; tartary buckwheat
한국식품과학회지 2017 Jun; 49(03): 258 - 264