→ 한국식품과학회지2017 ; 49(03): 242-251
Anti-proliferative effect of methanolic extracts from Citrus junos seeds and seed oils on HT-29 human colon cancer cells and identification of their major bioactive compounds
유자(Citrus junos)씨와 유자씨 유지의 메탄올 추출물에 의한 HT-29 대장암 세포 생장 억제 효과 및 유효 성분 분석
Kyungeun Kim, Hyunnho Cho, Hana Jung, Hee Jae Lee, and Keum Taek Hwang*
Department of Food and Nutrition, and Research Institute of Human Ecology, Seoul National University
서울대학교 식품영양학과·생활과학연구소
The purpose of this study was to investigate the anti-proliferative effect of methanolic extracts from Citrus junos (yuja) seeds and yuja seed oils against HT-29 human colon cancer cells and to identify the key compounds responsible for this effect. Extracts from yuja seeds, yuja seed oil prepared using hexane, and cold-pressed yuja seed oil were prepared using 60% methanol (ES, EHO, and ECO, respectively). The key compounds in the extracts were determined using HPLC-MS. Among the extracts, EHO and ECO inhibited proliferation of HT-29 cells. EHO and ECO were fractionated using preparative LC and the bioactive compounds were determined. Five of the fractions showed a significant anti-proliferative effect and the main compounds in the fractions were isopimpinellin, bergapten, and ichangensin. These compounds showed anti-proliferative effects on HT-29 cells when treated individually, and ichangensin showed the highest anti-proliferative activity. These results suggest that these compounds may be responsible for the anti-cancer effect of EHO and ECO.
yuja seed oil, Citrus junos, limonoid, ichangensin, HT-29 cell
한국식품과학회지 2017 Jun; 49(03): 242 - 251