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한국식품과학회지
→ 한국식품과학회지2017 ; 49(02): 123-131
Monitoring of norovirus and indicator microorganisms from agricultural products and environmental samples in Korea
Ji Hyun Kang, Hye Mee Shim, and Kwang Yup Kim*
Department of Food Science and Biotechnology, Chungbuk National University
ABSTRACT
Norovirus causes frequent epidemic viral gastroenteritis in Korea. The team for the control of noroviral foodborne outbreaks (NOROTECL) executed a project to trace the cause of norovirus contamination in agricultural products and environmental samples to reduce norovirus outbreaks in Korea. Between January and November in 2015, the contaminations by norovirus and indicator microorganisms such as coliforms, Escherichia coil and male specific coliphage (MSC) were examined in 80 agricultural products, 80 soil samples, 78 human feces samples, 3 animal feces samples, 80 agricultural water samples and 80 river water samples. Semi-nested PCR and DNA sequencing revealed 18 genogroup I and 3 genogroup II noroviruses in a total of 18 samples. These noroviruses were validated by real-time (RT)-PCR analysis. For indicator microorganisms, coliform and E. coli were respectively detected in agricultural products (68, 1%), soils (88, 7%), human feces (44, 12.8%), animal feces (67, 67%), agricultural waters (74, 30%) and river waters (96, 51%). The MSC results revealed 14 positive samples.
KEYWORD
norovirus, male-specific coliphage, semi-nested PCR, real-time RT-PCR
한국식품과학회지 2017 Apr; 49(02): 123 - 131
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