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Food Science and Biotechnology
→ Food Science and Biotechnology 2017 ; 26(2): 357-362
Identifying the geographical origin of protected sea cucumbers (Apostichopus japonicus) in China using random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR)
Zhenyu Yun*, Zhao Sun, Haiyan Xu1, Zhihong Sun1, Yao Zhang, and Zhen Liu
Sub-Institute of Food and Agriculture Standardization, China National Institute of Standardization, Beijing 100191, China 1College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China
ABSTRACT
Dalian sea cucumber, Yantai sea cucumber, and Weihai sea cucumber, which belong to Apostichopus japonicus, are protected as geographical indications in China based on their high nutritional values and medical propertys. The 26 samples, including Dalian sea cucumbers (9 samples) in Liaoning province, Yantai sea cucumbers (9 samples), and Weihai sea cucumbers (8 samples) in Shandong province, were individually collected from the designated geographical sea areas and the genetic relationships and DNA polymorphisms were evaluated by random amplified polymorphic DNA technology and gene segments sequencing. The RAPD dendrogram showed that the genetic diversity of the three types of sea cucumbers was rich. The neighbor-joining tree shows that the genetic relationship of the samples from the adjacent sea areas is closer. It demonstrates that the gene characteristics of sea cucumbers from different sea areas were obvious and the genetic diversity analysis by RAPD-PCR can be used as a rapid method for geographical discrimination.
KEYWORD
sea cucumber, geographical origin, random amplified polymorphic DNA, genetic diversity
Food Science and Biotechnology 2017 ; 26(2): 357-362
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