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Food Science and Biotechnology
→ Food Science and Biotechnology 2017 ; 26(1): 15-19
Determination of benzo[a]pyrene in camellia oil via vortexassisted extraction using the UPLC-FLD method
Runhong Mo, Yanping Zhang, Zhanglin Ni, and Fubin Tang*
Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang, Zhejiang 311400, China
ABSTRACT
In this paper, vortex-assisted extraction using the ultraperformance liquid chromatography analysis method was performed to determine benzo[a]pyrene in camellia oil. Optimum results were obtained when 0.5 g of oil sample was used followed by vortex-assisted extraction for 10 min with 25 mL of acetonitrile. Chromatographic separation was performed on an Agilent ZORBAX Eclipse Plus C18 column (2.1mm×100mm, particle size 1.8 μm). The optimum mobile phase comprised 70% acetone and 30% water. The detection limit of benzo[a]pyrene was 0.2 μg/kg. The recoveries were in the range of 81.0–97.0%. The proposed method was simple and fast, and it provided high throughput in the determination of benzo[a]pyrene in an oil matrix sample.
KEYWORD
benzo[a]pyrene, vortex-assisted extraction, UPLC, camellia oil
Food Science and Biotechnology 2017 ; 26(1): 15-19
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