→ 한국식품과학회지2021 ; 53(5): 570-577
Immunostimulatory activity and structural characteristics of neutral polysaccharides isolated from ginseng leaves fermented by Cordyceps sinensis
동충하초균으로 발효한 인삼잎에서 분리한 중성다당의 면역활성 및 구조적 특성
Ha Young Cha1, Hye-Ryung Park1,2, and Kwang-Soon Shin1,*
1Department of Food Science and Biotechnology, Kyonggi University 2Graduate School of Biotechnology, and College of Life Science, Kyung Hee University
차하영1 · 박혜령1,2 · 신광순1,*
1경기대학교 식품생물공학과, 2경희대학교 한방생명공학과
This study elucidated the biological activities and structural characteristics of polysaccharides isolated from ginseng leaves fermented using Cordyceps sinensis (GLF). GLF comprised at least 18 glycosyl linkages, including 4-linked glucose residues (24.0%). To characterize the neutral polysaccharides in GLF, it was further fractionated by anion exchange chromatography, and the unabsorbed fraction (GLF1) was isolated. Peritoneal macrophages stimulated with GLF1 produced various cytokines in a dose-dependent manner. The properties and activities of the four subfractions (PHI, PHIA1-PHIA3) obtained after sequential enzymatic digestion were examined. PHI and PHIA3 primarily comprised glucose, whereas PHI exhibited an iodine-color reaction. Furthermore, the PHIA1-3 fractions indicated that cytokine production was completely inhibited. These results suggest at the immune activities of GLF1 may be due to the α-(1→4)-glucan branched at the C(O)6 position, which was produced by C. sinensis.
Ginseng leaves, Cordyceps sinensis, fermentation, structural characteristics, α-glucan
한국식품과학회지 2021 Oct; 53(5): 570 - 577