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Food Science and Biotechnology
→ Food Science and Biotechnology 2021 ; 30(9): 1225-1231
Ultra-fast PCR method for the distinguishing between Miichthys miiuy and Sciaenops ocellatus
Yewon Hong1 • Jung Ju Kim1 • Yeon-Cheol Yu1 • Hyung Soo Kim1 • Guiim Moon1 • Eun Mi Park1
1 New Hazardous Substance Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong 28159, Chungcheongbuk-do, Korea
ABSTRACT
The mi-iuy croaker Miichthys miiuy has immense commercial value in the Republic of Korea. The red drum Sciaenops ocellatus is widely produced by aquaculture, although its price is approximately 25% that of M. miiuy. S. ocellatus has black spots on its tail, enabling it to be distinguished from M. miiuy based on appearance. However, identifying S. ocellatus after simple processing steps, such as skin removal and dicing, is difficult. Certain traders misrepresent and sell S. ocellatus as M. miiuy or cultured M. miiuy for illegal economical gain. Therefore, an accurate and rapid identification method is required to distinguish between M. miiuy and S. ocellatus in the field. Here, a method for rapid field identification was developed based on species-specific primers using a portable ultra-fast PCR instrument. The ultra-fast real-time PCR method can complete the entire analytical procedure, including DNA isolation, amplification, and detection, within 30 min, thus maintaining the accuracy of identifying M. miiuy and S. ocellatus products on site. Forty-nine commercial products were tested, and all samples were successfully identified. Thus, the developed method is rapid, efficient tool for ensuring consumer protection.
KEYWORD
Economically motivated adulteration · fishery products · Species-specific PCR · Miichthys miiuy · Ultrafast real-time PCR
Food Science and Biotechnology 2021 ; 30(9): 1225-1231
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