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한국식품과학회지
→ 한국식품과학회지2021 ; 53(4): 423-433
Protective effect of Eucommia ulmoides oliver leaves against PM2.5-induced oxidative stress in neuronal cells in vitro
미세먼지(PM2.5)로 유도된 산화적 스트레스에 대한 두충(Eucommia ulmoides Oliver) 잎의 in vitro 뇌 신경세포 보호 효과
Min Ji Kim1, Jin Yong Kang1, Seon Kyeong Park1, Jong Min Kim1, Jong Hyun Moon1, Gil Han Kim1, Hyo Lim Lee1, Hye Rin Jeong1, and Ho Jin Heo1,*
1Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University
김민지1 · 강진용1 · 박선경1 · 김종민1 · 문종현1 · 김길한1 · 이효림1 · 정혜린1 · 허호진1,*
1경상국립대학교 응용생명과학부(BK21), 농업생명과학연구원
ABSTRACT
This study was performed to examine the neuroprotective effect of the ethyl acetate fraction from Eucommia ulmoides oliver leaf (EFEL) on PM2.5-induced cytotoxicity. EFEL had higher total phenolic and flavonoid contents than the other fractions. In ABTS and DPPH radical scavenging activities, the IC50 of EFEL was measured as 212.80 and 359.13 μg/mL, respectively. To investigate the neuroprotective effect of EFEL, MTT and DCF-DA assays were performed on HT22, MC-IXC, and BV-2 cells. EFEL effectively decreased PM2.5-induced intercellular reactive oxygen species (ROS) content and inhibited PM2.5-induced cell death. In the results of protein expression related to cellular cytotoxicity on microglial cells (BV-2), EFEL had an improvement effect on cell apoptosis and inflammatory pathways. Rutin and chlorogenic acid were identified as the main physiological compounds. Moreover, it was expected that EFEL, including rutin and chlorogenic acid, could be functional food substances with neuroprotective effects against PM2.5-induced oxidative stress.
KEYWORD
Eucommia ulmoides oliver leaf, PM2.5, oxidative stress, neuroprotection, apoptosis
한국식품과학회지 2021 Aug; 53(4): 423 - 433
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