HOME | LOGIN | MEMBER JOIN | CONTACT US | ENGLISH

Food Science and Biotechnology
→ Food Science and Biotechnology 2021 ; 30(7): 959-969
Inulinase from Rhodotorula mucilaginosa: immobilization and application in the production of fructooligosaccharides
Geise Camila de Araujo Ribeiro1 • Pedro Fernandes2,3 • Dayse Alessandra Almeida Silva4 • Hugo Neves Branda˜o4 • Sandra Aparecida de Assis1
1 Laboratory of Enzymology and Fermentation Technology, Department of Health, State University of Feira de Santana, Av Transnordestina, Km 0, BR 116, Feira de Santana, Bahia 44036-900, Brazil 2 DREAMS and Faculty of Engineering, Luso´fona University, Lisboa, Portugal 3 Department of Bioengineering, IBB - Institute for Bioengineering and Biosciences, Instituto Superior Te´cnico, University of Lisbon, Lisboa, Portugal 4 Laboratory of Vegetal Bioprospection, Department of Health, State University of Feira de Santana, Av Transnordestina, Km 0, BR 116, Feira de Santana, Bahia 44036-900, Brazil
ABSTRACT
The crude extract containing inulinase from Rhodotorula mucilaginosa was obtained by submerged fermentation. Inulinase was immobilized on chicken eggshell by physical adsorption and covalent crosslinking, using glutaraldehyde as a crosslinking reagent, and Celite by adsorption. Fructooligosaccharides production was performed using immobilized inulinase (5%, w/v) and inulin substrate solution under experimental conditions evaluated through Doehlert experimental design. The production of inulinase was optimized for concentrations of D-glucose and yeast extract at 12.5 and 0.5 g/L, respectively, resulting in an optimal activity of 0.62 U. The optimal pH and temperature for enzyme activity were 8.0 and 75 °C, respectively, leading to an optimal activity of 3.54 U. The highest immobilization efficiency (46.27%) was obtained upon immobilization on Celite. Immobilization by adsorption to eggshell allowed for specific activity of 4.15 U/g, and adsorption to Celite resulted in specific activity of 3.70 U/g. The highest titer in fructooligosaccharides was obtained with an initial inulin concentration of 250 g/L (25%, w/v), and a reaction time of 16 h. Hence, immobilized inulinase proved to be a promising catalyst for fructooligosaccharides production since the formulation is performed through a simple, low-cost, and large-scale applicable methodology.
KEYWORD
Hydrolase · Immobilization · Inulin · Optimization · Yeast
Food Science and Biotechnology 2021 ; 30(7): 959-969
List