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Food Science and Biotechnology
→ Food Science and Biotechnology 2021 ; 30(4): 565-570
Detection of GM Canola MS11, DP-073496-4, and MON88302 events using multiplex PCR coupled with capillary electrophoresis
Do-Geun Lee1 • Ji-Eun Park1 • Mi-Ju Kim1 • Hyun-Joong Kim2 • Hae-Yeong Kim1
1 Institute of Life Sciences & Resources and Department of Food Science & Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea 2 Department of Food Engineering, Mokpo National University, Muan 58554, Republic of Korea
ABSTRACT
As of 2020, 11 GM canola events have been authorized as food for humans in Korea. However, there are no simultaneous multiplex detection methods for 3 GM canola events (DP-073496-4, MON88302, and MS11). Thus, we established the multiplex polymerase chain reaction (PCR) method coupled with capillary electrophoresis to detect 3 GM canola events. To verify the specificity of event-specific primers, various GM crops of 3 GM soybean events, 6 GM maize events, 2 GM cotton events and 11 GM canola events were prepared. The limit of detection of the developed multiplex PCR was approximately 0.0125% for 3 GM canola events. Certified GM canola and stacked events were analyzed to validate the developed multiplex PCR. This study focuses on establishing multiplex PCR coupled with capillary electrophoresis for newly approved GM canola events and contributes to efficient monitoring GM canola samples in Korea.
KEYWORD
Genetically modified canola · Event-specific detection · Multiplex polymerase chain reaction · Monitoring · Capillary electrophoresis
Food Science and Biotechnology 2021 ; 30(4): 565-570
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