→ 한국식품과학회지2021 ; 53(1): 34-39
Anti-cancer effects of Salicornia herbacea extract in OVCAR-3 cells by induction of apoptosis pathway
함초 추출물의 세포사멸 유도에 의한 난소암세포에서 항암 효과
Yu Ri La1,2†, You Rim Lee1,2†, Dong Seok Lee1,2, Soo Hwan Kim3, and Hyeong Seon Lee4,*
1Department of Smart Foods and Drugs, Inje University 2Department of Biomedical Laboratory Science, Inje University 3Department of Biomedical Laboratory Science, Gimcheon University 4Department of Biomedical Laboratory Science, Jungwon University
라유리1,2† ·이유림1,2† ·이동석1,2 ·김수환3 ·이형선4,*
1인제대학교 일반대학원 식의약생명공학과, 2인제대학교 의생명과학대학 임상병리학과, 3김천대학교 헬스케어대학 임상병리학과, 4중원대학교 의료보건대학 임상병리학과
This study investigated the anti-cancer effects of Salicornia herbacea L. fractions in human ovarian cancer cells (OVCAR-3). S. herbacea powder was extracted with 95% EtOH and sequentially fractionated with hexane, dichloromethane (DCM), ethyl acetate, butanol, and H2O. Further, the growth inhibitory effects of the six fractions were determined using the MTS assay. The DCM fraction dramatically decreased cell viability. Similarly, the cell cycle was arrested at the sub- G1 phase in DCM-treated cells. To confirm apoptosis, the cells were stained with annexin V/FITC-PI solution. Total, early, and late apoptotic cells were significantly increased in the DCM fraction. The mRNA expression of Bcl-2 was reduced, whereas the pro-apoptotic factors Bax and Bak were increased in DCM fraction-treated cells. These results indicated that the DCM fraction in S. herbacea exhibited strong apoptotic effects through the p53-dependent signaling pathway.
Salicornia herbacea L., apoptosis, OVCAR-3 cells, DCM fraction
한국식품과학회지 2021 Feb; 53(1): 34 - 39