→ 한국식품과학회지2020 ; 52(6): 587-594
Analysis of glucosinolates and their metabolites from napa cabbage (Brassica rapa subsp. Pekinensis) and napa cabbage kimchi using UPLC-MS/MS
UPLC-MS/MS를 이용한 배추와 배추김치의 글루코시놀레이트 및 대사체 분석
Jaecheol Kim1,2, Hyo Sun Park3 , Keum Taek Hwang1,2, BoKyung Moon3 , and Suna Kim4,*
1Department of Food and Nutrition, and Research Institute of Human Ecology, Seoul National University 2BK21 POUR Education and Research Team for Sustainable Food & Nutrition, Seoul National University 3Department of Food and Nutrition, Chung-Ang University 4Division of Human Ecology, College of Natural Science, Korea National Open University
김재철1,2·박효순3 ·황금택1,2·문보경3 ·김선아4,*
1서울대학교 생활과학대학 식품영양학과·생활과학연구소, 2서울대학교 4단계 BK21 지속가능 식품영양 교육연구팀 3중앙대학교 생명공학대학 식품공학부 식품영양전공, 4한국방송통신대학교 자연과학대학 생활과학부 식품영양학전공
t In this study, we analyzed glucosinolates and their metabolites in the inner and outer parts of napa cabbage (NC; Brassica rapa subsp. pekinensis) and napa cabbage kimchi (NKC) using UPLC-ESI-MS/MS. In the extracts from NC and NKC, glucobrassicanapin (m/z 386), glucoalyssin (m/z 450), glucobrassicin (m/z 447), 4-methoxyglucobrassicin (m/z 477), and neoglucobrassicin (m/z 477) were detected using the MS scan mode ([M-H]− ), and gluconapin (m/z 372→97), progoitrin (m/z 388→97), glucoiberin (m/z 422→97), 4-methoxyglucobrassicin (m/z 477→97), and neoglucobrassicin (m/z 477→447) were detected using the MS/MS MRM mode ([M-H]− ). Ascorbigen (m/z 306→130) and indole-3- carboxaldehyde (I3A; m/z 146→118), which were metabolites of glucobrassicins, were detected using the MS/MS MRM ([M+H]+ ) mode. The peak intensities of ascorbigen in the extract from the inner and outer parts of NC were significantly higher than those of the NKC extract (p<0.05); however, there was no significant difference in I3A peak intensity between the NC and NKC extracts.
ascorbigen, napa cabbage, glucosinolate, kimchi, UPLC-MS/MS
한국식품과학회지 2020 Dec; 52(6): 587 - 594