→ 한국식품과학회지2020 ; 52(6): 580-586
Validation of a trienzyme-Lactobacillus casei method for folate analysis in fishery resources consumed in the Korean diet
Trienzyme과 Lactobacillus casei를 이용한 국내 수산 자원의 엽산 분석 및 유효성 검증
Bomi Jeong1, Ki-Ho Nam2, Yeon-Kye Kim2, and Jiyeon Chun1,*
1Department of Food Science and Technology, Sunchon National University 2Food Safety and Processing Research Division, National Institute of Fisheries Science
정보미1 ·남기호2 ·김연계2 ·천지연1,*
1순천대학교 식품공학과, 2국립수산과학원 식품위생가공과
Fishery resources have been widely consumed as protein- and vitamin-rich food sources in the Korean diet. However, information regarding their vitamin levels is extremely limited. In this study, trienzyme-Lactobacillus casei method was validated and used to determine the folate contents in fishery foods. The trienzyme-L. casei method for folate analysis showed excellent accuracy (85.2 to 95.3% recovery) and precision (repeatability 1.4% RSD and reproducibility 2.4% RSD). Folate contents of 20 fish foods (4 fish, 3 crustaceans, 3 sea algae, 3 cephalopods, 4 shellfish, and 3 others) ranged from 1.75 to 97.98 µg/100 g. Furthermore, we found that the folate content in seaweed fusiforme was the highest, followed by gulfweed (69.73 µg/100 g). Folate analysis using the trienzyme-L. casei method was determined excellent based on the z-score of −0.3 in the Food Analysis Performance Assessment Scheme test. Analytical and method validation data generated in this study could be used to update the national food composition table on vitamin B9 in Korean fishery resources.
fishery resources, folate, validation, database, vitamin
한국식품과학회지 2020 Dec; 52(6): 580 - 586