→ 한국식품과학회지2020 ; 52(4): 385-395
3,4-Dihydroxytoluene suppresses UVB-induced wrinkle formation by inhibiting Raf-1
Sang-Hee Park1 and Nam Joo Kang1,2,*
1School of Food Science and Biotechnology, Kyungpook National University 2Department of Integrative Biology, Kyungpook National University
This study examined the effect of 3,4-dihydroxytoluene (DHT) on UVB-induced photoaging and determined its molecular mechanisms, using HaCaT human keratinocytes and SKH-1 hairless mice. DHT suppressed UVB-induced matrix metalloproteinase-1 (MMP-1) expression in HaCaT cells. in vivo data from mouse skin supported that DHT decreased UVBinduced wrinkle formation, epidermal thickness, and matrix metalloproteinase-13 (MMP-13) expression. DHT appeared to exert its anti-aging effects by suppressing UVB-induced Raf-1 kinase activity and subsequent attenuation of UVB-induced phosphorylation of MEK, ERK, and p90RSK in HaCaT cells. in vitro and in vivo pull-down assays revealed that DHT bound with Raf-1 in ATP-noncompetitive manner. Overall, DHT appears to anti-photoaging effects in vitro and in vivo through the suppression of Raf-1 kinase activity and may have potential as a treatment for the prevention of skin aging.
3,4-dihydroxytoluene, Raf-1, UVB, photoaging
한국식품과학회지 2020 Aug; 52(4): 385 - 395