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Food Science and Biotechnology
→ Food Science and Biotechnology 2020 ; 29(9): 1251-1260
In vivo the antioxidative extract of Averrhoa carambola Linn. leaves induced apoptosis in Ehrilch ascites carcinoma by modulating p53 expression
Ayesha Siddika1,2 • Tasnim Zahan1 • Lipy Khatun1 • Md. Rowshanul Habib1 • Md. Abdul Aziz1 • A. R. M. Tareq3 • Md. Habibur Rahman1 • Md. Rezaul Karim1
1 Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi 6205, Bangladesh 2 Institute of Tissue Banking and Biomaterial Research, Atomic Energy Research Establishment (AERE), Savar, Dhaka 1349, Bangladesh 3 Environmental and Organic Laboratory, Chemistry Division, Atomic Energy Centre, Dhaka 1349, Bangladesh
ABSTRACT
This study was designed to evaluate the antioxidant activity of methanol extract of Averrhoa carambolla Linn. leaves (MELA) using DPPH and ABTS.+ free radical scavenging assays whereas its antineoplastic effect against Ehrlich ascites carcinoma (EAC) was assed using viable cell count, life span, body weight gain and hematological parameters of experimental mice. Results showed that rich phenolic and flavonoid content of MELA had moderate dose dependent free radical scavenging activity (IC50: 62.0 µg/mL for DPPH. and 6.0 µg/mL for ABTS.+). In vivo antineoplastic assay, MELA significantly (P<0.05) decreased viable cells and body weight gain, increased the survival time and restored altered hematological profiles of cancer cell bearing mice. Fluorescence microscopic view of EAC cells derived from MELA-treated group showed apoptotic characteristics and this observation was also supported by overexpression of proapoptotic genes coding p53 and Bax proteins in treated cancer cells. The anti-apoptotic genes coding Bcl-2 protein was also absent in treated EAC cells as compared with the control. Moreover, phytochemical profiles of MELA as identified by GC/MS analysis are also consistent with its activities.
KEYWORD
Averrhoa carambolla · Leaves · Antioxidant · Antineoplastic · Apoptosis
Food Science and Biotechnology 2020 ; 29(9): 1251-1260
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