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Food Science and Biotechnology
→ Food Science and Biotechnology 2020 ; 29(9): 1240-1250
Resistance of pathogenic biofilms on glass fiber filters formed under different conditions
Hana Song1 • Sun-Young Lee1
1Department of Food and Nutrition, Chung-Ang University, 4726, Seodong-daero, Anseong-si, Gyeonggi-do, Republic of Korea
ABSTRACT
This study investigated the survivals of two pathogens (Escherichia coli O157:H7 and Staphylococcus aureus) in different adhered forms on glass fiber filters (GFFs) at 43 and 68% relative humidity (RH). Efficacies of chemical sanitizers at reducing pathogenic biofilms on GFFs were also evaluated. Inoculated GFFs were incubated at 28 °C in TSB (type I), on TSA (type II), or on TSA under 100% RH (type III) to produce biofilms. When GFFs were incubated at 43 or 68% RH for 7 days, type III biofilms were less than 2 log10 CFU/filter reduction whereas type I and type II biofilms were 4–6 log10 CFU/filter reduction. Additionally, type III biofilms were highly resistant to sanitizing treatment compared than other biofilms (type I and II). Therefore, the method to produce biofilms used in this study could be used to produce highly resistance pathogenic biofilms in the laboratory for related experiments.
KEYWORD
Biofilm · Glass fiber filter · Adhered form · Chemical sanitizer · Relative humidity
Food Science and Biotechnology 2020 ; 29(9): 1240-1250
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