→ 한국식품과학회지2020 ; 52(3): 274-283
Industrial potential of domestic Zanthoxylum piperitum and Zanthoxylum schinifolium: Protective effect of both extracts on high glucose-induced neurotoxicity
국내산 초피와 산초의 산업적 활용 가능성: 고당으로 유도된 뇌신경세포 독성에 대한 추출물의 보호 효과
Hye Ju Han1, Seon Kyeong Park1, Min Ji Kim1, Jun Woo An2, Se Jin Lee2, Jin Yong Kang1, Jong Min Kim1, and Ho Jin Heo1,2,*
1Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University 2Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University
1경상대학교 응용생명과학부(BK21 Plus), 농업생명과학연구원, 2경상대학교 농업생명과학대학 식품공학과
This study focused on the in vitro investigation of antioxidant and anti-diabetic activities, along with neuroprotection against high glucose-induced cytotoxicity, in order to evaluate the physiological effects of Zanthoxylum piperitum and Zanthoxylum schinifolium. The highest total phenolic content was measured in the 40% ethanolic extracts of Zanthoxylum piperitum (EZP) and Zanthoxylum schinifolium (EZS). The in vitro EZP antioxidant activity showed a relatively higher ABTS/DPPH radical scavenging activity and malondialdehyde inhibitory effect than that of EZS. The EZP inhibited carbohydrate hydrolysis (α-glucosidase and α-amylase) more efficiently than EZS in anti-diabetic tests. However, EZS showed a more efficient inhibition of advanced glycation end-products formation than EZP. In addition, both EZP and EZS effectively protected human-derived neuronal cells from high glucose-induced cytotoxicity. Finally, the physiological compounds were analyzed using UPLC IMS-QTOF/MSE, and the main EZP (quercetin-3-O-glucoside and 3-caffeoylquinic acid) and EZS (5-caffeoylquinic acid) compounds were identified as phenolic compounds.
diabetes, high glucose, neuroprotection, Zanthoxylum piperitum, Zanthoxylum schinifolium
한국식품과학회지 2020 Jun; 52(3): 274 - 283