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Food Science and Biotechnology
→ Food Science and Biotechnology 2020 ; 29(7): 939-951
Comparative analysis of transcriptomes revealed the molecular mechanism of development of Tricholoma matsutake at different stages of fruiting bodies
Xian Tang1 • Xiang Ding2 • Yi-ling Hou1
1 Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), College of Life Sciences, China West Normal University, 1# Shida Road, Nanchong 637009, Sichuan Province, China 2 College of Environmental Science and Engineering, China West Normal University, 1# Shida Road, Nanchong 637009, Sichuan Province, China
ABSTRACT
The purpose of the study is to investigate the molecular mechanisms of development of Tricholoma matsutake fruiting body at the primordial stage (TM-1), the intermediate stage (TM-2) and the mature stage (TM-3) using RNA-Seq sequencing technology. The analysis of gene expression level revealed that the Spn2 and Eef1a1 gene were the key genes in the primordial stage of T. matsutake by regulating cytokinesis, protein synthesis, and cell growth. And the Ubc, Atp6, Cytb, and Pth2 gene were the key genes in the mature stage of T. matsutake by regulating energy metabolism and protein synthesis. Differential expression genes (DEGs) analysis results showed that Cdc28, Rad53, Dun1, Pho85 and Pho81 were the key DEGs regulating cell cycle genes of T. matsutake from primordial stage to intermediate stage. And APC, Cyr1, Cdc45, Spo11 and Rec8 genes were the key DEGs for the meiosis and sporogenesis of T. matsutake from the intermediate stage to the mature stage.
KEYWORD
Tricholoma matsutake ㆍ Molecular mechanism ㆍ Development ㆍ RNA-sequencing ㆍ Transcriptome
Food Science and Biotechnology 2020 ; 29(7): 939-951
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