→ 한국식품과학회지2019 ; 51(4): 336-342
Roles of sugar chains in immunostimulatory activity of the polysaccharide isolated from Angelica gigas
참당귀에서 분리한 다당의 면역활성에 대한 당쇄의 역할
Kwang-Soon Shin1
1Department of Food Science and Biotechnology, Kyonggi University
1경기대학교 식품생물공학과
To elucidate structure-function relationship of polysaccharide from Angelica gigas, the AGE-2c-I was purified by two successive chromatography steps. AGE-2c-I showed a potent anti-complementary activity in a dose-dependent manner. AGE-2c-I with a molecular weight of 140 kDa comprised four monosaccharides and 13 glycosyl linkages, and strongly reacted with β-glucosyl Yariv reagent. For the fine structure analysis of AGE-2c-I, it was sequentially digested by exo-arabinofuranosidase and endo-galactanase. The results indicated that AGE-2c-I was a typical RG-I polysaccharide with side chains such as highly branched α-arabinan, β-(1→4)-galactan and arabino-β-3,6-galactan. To characterize the active moiety of AGE-2c-I, the anti-complementary activities of AGE-2c-I and its subfractions were assayed. It was observed that the anti-complementary activity of AGE-2c-I was due to the entire structure that resembled RG-I. In addition, arabino-β-3,6-galactan side chain (GN-I) in AGE-2c-I probably plays a crucial role in the anti-complementary activity, whereas α-arabinan side chain (AFN-I) consisting of 5-linked Araf and 3,5-branched Araf partially contributes to the activity.
Angelica gigas, polysaccharide, rhamnogalacturonan-I, anti-complementary activity, structure-function relationship
한국식품과학회지 2019 Aug; 51(4): 336 - 342