→ 한국식품과학회지2019 ; 51(4): 301-308
Validation of analytical method and antioxidant properties of Eriobotrya japonica Lindl. Leaf extract according to extraction solvent
추출용매 조건에 따른 비파 잎 추출물의 항산화 활성 및 유효성분의 분석법 밸리데이션
Hyun-Hee Kim1,†, Mi-Ra Heo2,†, Songmi Lee3, and Soon-Ho Yim1,*
1Department of Pharmaceutical Engineering, Dongshin University, 2Department of Cosmetology, Dongshin University, 3Department of Food and Nutrition, Dongshin University
1동신대학교 제약공학과, 2동신대학교 뷰티미용학과, 3동신대학교 식품영양학과
The antioxidant properties of Eriobotrya japonica leaf extract were investigated using DPPH and ABTS radical scavenging assay. The 80% ethanol extract of leaves (IC50 values for DPPH and ABTS were 13.9 and 10.9 µg/mL, respectively) and young leaves (IC50 values for DPPH and ABTS were 20.7 and 17.3 µg/mL, respectively) showed high radical scavenging activity. Additionally, the quantitative method for estimation of ellagic acid and chlorogenic acid from E. japonica leaves was optimized by HPLC/DAD. This method showed high linearity of the calibration curve with a coefficient of correlation (R2) equal to 0.999. The LOD values for ellagic acid and chlorogenic acid were 2.35 and 0.73 µg/mL, respectively, whereas LOQ values were 7.13 and 2.22 µg/mL, respectively. Recovery of the two compounds was 99.7-108.0% with RSD values less than 5.31%. These results suggest that 80% ethanol extract of E. japonica leaves could serve as a potential source of natural antioxidant for us in various industrial applications.
Eriobotrya japonica, antioxidant, ellagic acid, chlorogenic acid, validation
한국식품과학회지 2019 Aug; 51(4): 301 - 308