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한국식품과학회지
→ 한국식품과학회지2019 ; 51(4): 301-308
Validation of analytical method and antioxidant properties of Eriobotrya japonica Lindl. Leaf extract according to extraction solvent
추출용매 조건에 따른 비파 잎 추출물의 항산화 활성 및 유효성분의 분석법 밸리데이션
Hyun-Hee Kim1,†, Mi-Ra Heo2,†, Songmi Lee3, and Soon-Ho Yim1,*
1Department of Pharmaceutical Engineering, Dongshin University, 2Department of Cosmetology, Dongshin University, 3Department of Food and Nutrition, Dongshin University
김현희1,†·허미라2,†·이송미3·임순호1,*
1동신대학교 제약공학과, 2동신대학교 뷰티미용학과, 3동신대학교 식품영양학과
ABSTRACT
The antioxidant properties of Eriobotrya japonica leaf extract were investigated using DPPH and ABTS radical scavenging assay. The 80% ethanol extract of leaves (IC50 values for DPPH and ABTS were 13.9 and 10.9 µg/mL, respectively) and young leaves (IC50 values for DPPH and ABTS were 20.7 and 17.3 µg/mL, respectively) showed high radical scavenging activity. Additionally, the quantitative method for estimation of ellagic acid and chlorogenic acid from E. japonica leaves was optimized by HPLC/DAD. This method showed high linearity of the calibration curve with a coefficient of correlation (R2) equal to 0.999. The LOD values for ellagic acid and chlorogenic acid were 2.35 and 0.73 µg/mL, respectively, whereas LOQ values were 7.13 and 2.22 µg/mL, respectively. Recovery of the two compounds was 99.7-108.0% with RSD values less than 5.31%. These results suggest that 80% ethanol extract of E. japonica leaves could serve as a potential source of natural antioxidant for us in various industrial applications.
KEYWORD
Eriobotrya japonica, antioxidant, ellagic acid, chlorogenic acid, validation
한국식품과학회지 2019 Aug; 51(4): 301 - 308
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