→ 한국식품과학회지2019 ; 51(2): 188-191
Improvement of the electro-transformed cell yield for Bifidobacterium sp. with large DNA
Min Ju Park1, Myeong Soo Park2,3,*, and Geun Eog Ji1,2,*
1Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University, 2Research Center, BIFIDO Co., Ltd., 3Department of Hotel Culinary Arts, Yeonsung University
In this study, the poor electro-transformant yield of large DNA in bifidobacteria was improved by increasing the DNA concentration, which was amplified by enhancing electroporation conditions: treating the cell wall weakening agent and cell membrane permeabilizing molecule as well as changing the electrical parameter. In the enhanced conditions, the electroporation frequency increased from 15 to 71 times compared to the initial conditions at the same DNA concentration. As the DNA concentration increased, the difference in the electroporation frequency between the two conditions became greater, and the curve of the enhanced conditions seemed to be saturated with a DNA concentration over 4 μg. The present study provided a clue to the recovery of the electroporation frequency with large DNA and formulated the relationship between the DNA concentration, the DNA size and the electroporation frequency in bifidobacteria. Therefore, this study will contribute to the expansion of molecular genetic studies of bifidobacteria.
large dna, electroporation, bifidobacteria
한국식품과학회지 2019 Apr; 51(2): 188 - 191