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한국식품과학회지
→ 한국식품과학회지2019 ; 51(2): 152-159
Immunomodulatory and anti-metastatic activities of a crude polysaccharide isolated from Korean apple vinegar
한국산 사과식초에서 분리한 다당의 면역 및 항전이 활성
Han Wool Kim1 and Kwang-Soon Shin1,*
1Department of Food Science and Biotechnology, Kyonggi University
김한울1·신광순1,*
1경기대학교 식품생물공학과
ABSTRACT
To characterize new physiologically active components in Korean apple vinegar, a crude polysaccharide (KAV-0) was prepared by precipitation with 80% (v/v) ethanol. KAV-0 mainly comprises 38.2% mannose, 19.1% galactose and 14.3% glucose. In an in vitro cytotoxicity analysis, KAV-0 promoted the proliferation of peritoneal macrophages and RAW 264.7 cells in a dose-dependent manner, and showed no cytotoxicity in B16-BL6 melanoma cells. Murine peritoneal macrophages and RAW 264.7 cells stimulated by KAV-0 produced various cytokines such as interleukin (IL)-6, IL-12, and tumor necrosis factor (TNF)-α, and nitric oxide (NO). Intravenous (i.v.) administration of KAV-0 significantly augmented NK cell cytotoxicity against Yac-1 tumor cells. In experimental lung metastasis caused by B16-BL6 melanomas, prophylactic i.v. administration of KAV-0 at a dosage of 1,000 μg/mouse inhibited lung metastasis by 53.0%. These results suggest that the crude polysaccharide (KAV-0) isolated from Korean apple vinegar has a considerably high anti-metastatic activity and immunomodulatory activities beneficial to human health.
KEYWORD
apple vinegar, immunostimulatory activity, anti-metastasis, polysaccharide, natural killer cell
한국식품과학회지 2019 Apr; 51(2): 152 - 159
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