→ 한국식품과학회지2019 ; 51(2): 141-146
Antiangiogenic activity of non-aqueous fraction from Sparassis crispa extract in human umbilical vein endothelial cells
혈관내피세포에서 꽃송이버섯(Sparassis crispa) 소수성 추출물의 항혈관신생 활성
Jang Mi Han1, So Youn Gong1, Jae Kyung Sohng1, Yue Jai Kang2,*, and Hye Jin Jung1,*
1Department of Pharmaceutical Engineering & Biotechnology, Sun Moon University, 2Department of Aquatic Life and Medical Sciences, Sun Moon University
1선문대학교 제약생명공학과, 2선문대학교 수산생명의학과
Sparassis crispa is an edible mushroom that is distributed in Korea, Japan, Europe, and North America. It exerts various biological activities such as immunopotentiation, anti-diabetic, anti-cancer, and anti-inflammatory effects. Recently, we separated the health functional non-aqueous fraction from the chloroform extract of S. crispa (SCF4). In this study, we evaluated the antiangiogenic activity of SCF4 in human umbilical vein endothelial cells (HUVECs). SCF4 effectively inhibited vascular endothelial growth factor (VEGF)-induced cell growth at concentrations (5-25 μg/mL) showing no cytotoxic effects. SCF4 inhibited VEGF-induced invasiveness and tube formation ability, which are in vitro angiogenic features of HUVECs, in a dose-dependent manner. In addition, SCF4 markedly suppressed in vivo angiogenesis of chorioallantoic membrane from growing chick embryos without cytotoxicity. Furthermore, SCF4 downregulated the phosphorylation of VEGFR2, AKT, and ERK1/2, which are major angiogenic signal mediators. These results suggest that SCF4 inhibited angiogenesis by suppressing the VEGFR2 signaling pathways without cytotoxicity.
Sparassis crispa, non-aqueous fraction, angiogenesis, HUVECs, VEGFR2 signaling
한국식품과학회지 2019 Apr; 51(2): 141 - 146