→ 한국식품과학회지2019 ; 51(2): 103-108
Determination the optimum extraction method for saponin lancemasides in Codonopsis lanceolata
더덕 사포닌인 lancemasides의 최적 추출 방법 구명
Min Ju Lee1, Ju Hee Nam1, In Eeok Um1, Chang Keun Kang2,3, and Il Rae Rho1,3,*
1Department of Agronomy, Gyeongsang National University, 2College of Veterinary Medicine, Gyeongsang National University, 3Institute of Agriculture and Life Science, Gyeongsang National University
1경상대학교 농학과, 2경상대학교 수의학과, 3경상대학교 농업생명과학연구원
This study was conducted to select the optimal extraction method of codonopsis lanceolata saponin. To investigate the lancemasides content depending on each extraction method, various extractions were performed: reflux (methanol and butanol), hot water, as well as ultrasonic bath (40 kHz; continuous irradiation/interval irradiation) and ultrasonicator (20 kHz) extractions. From the result, the overall lancemasides content were the highest in ultrasonic bath (MeOH; continuous irradiation) extraction, followed by ultrasonic bath (water; continuous irradiation)>ultrasonic bath (MeOH; interval irradiation)>ultrasonicator (MeOH)>hot water>MeOH reflux>BuOH reflux extractions in that order. Sample drying method prior to ultrasonic bath extraction was more effective shade drying than freeze drying. Effective duration and temperature of extraction was 2 hr at 64°C. And ingredient change diverted from aster saponin Hb to lancemasides was identified by extraction condition such as extraction time and temperature.
fraction, mass, reflex extraction, ultrasonic bath, ultrasonicator
한국식품과학회지 2019 Apr; 51(2): 103 - 108