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Food Science and Biotechnology
→ Food Science and Biotechnology 2019 ; 28(2): 555-562
Protective effects of chebulic acid from Terminalia chebula Retz. against t-BHP-induced oxidative stress by modulations of Nrf2 and its related enzymes in HepG2 cells
Hye-Lim Jung1, Sung-Yong Yang1,2, Min Cheol Pyo1, Chung-Oui Hong1, Mi-Hyun Nam1,3, Jin-Won Lee4, Kwang-Won Lee1
1Department of Biotechnology, College of Life Science and Biotechnology, Korea University, 145 Anam-ro, Seongbukgu, Seoul 02841, Republic of Korea, 2Analytical Method Development Team, Samsung BIOEPIS, Incheon 21987, Republic of Korea, 3Department of Ophthalmology, University of Colorado School of Medicine, Aurora, CO 80045, USA, 4Faculty of Health and Food/Medicinal Plant, Suwon Women’s University, Suwon, Gyeonggi-do 16632, Republic of Korea
ABSTRACT
Although chebulic acid isolated from Terminalia chebular has diverse biological effects, its effects on the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and the expression of downstream genes have not been elucidated. The purpose of this research is to investigate the hepatoprotective mechanism of chebulic acid against oxidative stress produced by tert-butyl hydroperoxide (t-BHP) in liver cells. The treatment with chebulic acid attenuated cell death in t-BHP-induced HepG2 liver cells and increased intracellular glutathione content, upregulated the activity of heme oxygenase-1, and also increased the translocation of Nrf2 into the nucleus and Nrf2 target gene expression in a dose-dependent manner. The exposure of chebulic acid activated the phosphorylation of mitogen-activated protein kinases. The overall result is that chebulic acid has cytoprotective effect on t-BHP-induced hepatotoxicity in HepG2 cells through Nrf2-mediated antioxidant enzymes.
KEYWORD
Chebulic acid, Nuclear factor erythroid 2-related factor 2, Heme oxygenase-1, γ-Glutamate cysteine ligase, Mitogen-activated protein kinases
Food Science and Biotechnology 2019 ; 28(2): 555-562
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