→ 한국식품과학회지2019 ; 51(1): 24-28
In vitro hepatocyte inflammation by Ephedra sinica extracts
마황 추출물의 in vitro 간세포 염증반응 유도
Ilrang Kim1,*
1Department of Food and Nutrition, Ulsan College
김 일 낭1,*
1울산과학대학교 식품영양학과
In this study, the in vitro hepatotoxic mechanism of Ephedra sinica (ma-huang) was investigated by measuring the degree of cell death, secretion of cytokine, and fat accumulation by treating HepG2 cells with 70% ethanolic extracts of ma-huang. Cell death was observed at concentrations of around 5-100 μg/mL by treatment with ma-huang extracts (p<0.05). The secretion of interleukin 8 (IL-8) and macrophage colony-stimulating factor (M-CSF), which are inflammatory cytokines, were significantly promoted at concentrations of around 0.05-100 and 0.5-100 μg/mL, respectively (p<0.05). In this experiment, it was shown that the extracts of ma-huang stimulate the secretion of inflammatory cytokines, such as IL-8 and M-CSF, and lead to fat accumulation in the hepatocytes, thereby causing inflammation of the hepatocytes. Hepatotoxicity was observed at around 10-500 times lower concentration than the concentration required to cause serious toxicity, such as cell death, suggesting that hepatic toxicity (hepatitis) may be induced at a low dose.
Ephedra sinica (ma-huang), inflammation, interleukin-8, macrophage colony-stimulating factor, lipid accumulation
한국식품과학회지 2019 Feb; 51(1): 24 - 28