→ 한국식품과학회지2018 ; 50(06): 594-600
Isolation and characterization of bacteriophages for the control of Shiga Toxin-producing E. coli
시가 독소 생성 대장균의 제어를 위한 박테리오파지의 분리와 특성 분석
Ga-Yeon Lim1, Do Won Park1, Young-Duck Lee2, and Jong-Hyun Park1,*
1Department of Food Science and Biotechnology, College of BioNano Technology, Gachon University
2Department of Food Science and Engineering, Seowon University
1가천대학교 식품공학과, 2서원대학교 식품공학과
Shiga toxin-producing Escherichia coli (STEC) is an important pathogenic bacterium. To control STEC, the characteristics of the ECP33 and NOECP91 coliphages, which belong to the Myoviridae family, were analyzed. The host inhibition range for a total of 44 STEC strains was 45.5% for ECP33 and 65.9% for NOECP91. ECP33 and NOECP91 were relatively stable at 65°C, 50 ppm of sodium hyperchlorite, and a pH value of 4-10. However, the two phages were susceptible to a temperature of 70°C. NOECP91 was killed within 1 h after exposure to 30% ethanol, but ECP33 showed high tolerance even after exposure to 70% ethanol for 1 h. Interestingly, the inhibition of STEC growth according to the multiplicity of infection of 0.1 was confirmed until no growth was observed after 10 hours of culture with the phages. Therefore, the ECP33 and NOECP91 phages may be applied as a biological control agent for Shiga toxin-producing E. coli.
shiga toxin-producing E. coli, coliphage, host spectrum, stability, biocontrol
한국식품과학회지 2018 Dec; 50(06): 594 - 600