→ 한국식품과학회지2018 ; 50(05): 517-527
Protective effect of ethyl acetate fraction from Actinidia arguta sprout against high glucose-induced in vitro neurotoxicity
포도당으로 유도된 in vitro 뇌신경세포 독성에 대한 다래 순 아세트산에틸 분획물의 보호 효과
Seul Ki Yoo1, Seon Kyeong Park1, Jong Min Kim1, Jin Yong Kang1, Su Bin Park1, Hye Ju Han1, Chul-Wo Kim2, Uk Lee2, and Ho Jin Heo1,*
1Division of Applied Life Science (BK21 Plus), Institute of Agriculture and Life Science, Gyeongsang National University, 2Division of Special Forest Products, National Institute of Forest Science
유슬기1·박선경1·김종민1·강진용1·박수빈1·한혜주1·김철우2·이 욱2·허호진1,*
1경상대학교 응용생명과학부(BK21 plus), 농업생명과학연구원, 2국립산림과학원 산림소득자원연구과
The current study investigated in vitro anti-diabetic and neuroprotective effects of the ethyl acetate fraction in Actinidia arguta sprouts (EFAS), on H2O2 and high glucose-induced cytotoxicity in human neuroblastoma MC-IXC cells. EFAS had high total phenolic and total flavonoid contents. An assessment of 2,2'-azino-bis (3-ethylbenzothiazoline-6- sulfonic acid) radical scavenging activity of EFAS, as well as its potential for inhibiting malondialdehyde production, indicated that EFAS may possess significant antioxidant properties. EFAS exerted inhibitory effects on α-glucosidase via glycemic regulation which forms advanced glycation end products. In addition, EFAS exhibited significant acetylcholinesterase inhibitory effects. Moreover, EFAS displayed protective effects against H2O2 and high glucose-induced cell death, and inhibited the generation of reactive oxygen species in MC-IXC cells. Finally, the main physiological compound of EFAS was identified via high performance liquid chromatography as a rutin.
Actinidia arguta, antioxidant effect, anti-diabetic effect, neuroprotective effect, MC-IXC cell
한국식품과학회지 2018 Oct; 50(05): 517 - 527